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dc.contributor.authorZubkovs, Vitalijs
dc.contributor.authorWang, Hanxuan
dc.contributor.authorSchuergers, Nils
dc.contributor.authorWeninger, Astrid
dc.contributor.authorGlieder, Anton
dc.contributor.authorCattaneo, Stefano
dc.contributor.authorBoghossian, Ardemis A.
dc.identifier.citationNanoscale Advances, 2022en_US
dc.description.abstract"Single-walled carbon nanotubes (SWCNTs) emit photostable near-infrared (NIR) fluorescence that is conducive for optical glucose monitoring. Such SWCNT-based optical sensors often require the immobilization of proteins that can confer glucose selectivity and reactivity. In this work{,} we immobilize a glucose-reactive enzyme{,} glucose oxidase (GOx){,} onto SWCNTs using a N-(1-pyrenyl)maleimide (PM) crosslinker via thiol bioconjugation of engineered cysteine residues. We compare the conjugation of several glucose oxidase variants containing rationally-engineered cysteines and identify a D70C variant that shows effective bioconjugation. The bioconjugation was characterized through both absorption and fluorescence spectroscopy. Furthermore{,} we demonstrate an application for continuous glucose monitoring in the NIR-II optical region using the bioconjugated reaction solution{,} which shows a reversible response to physiological concentrations of glucose. Finally{,} we develop a miniaturized NIR-II reader to be used for cell cultures that require continuous glucose monitoring."en_US
dc.rightsCC0 1.0 Universal*
dc.subjectNear-infrared (NIR) fluorescenceen_US
dc.subjectOptical glucose monitoringen_US
dc.subjectSingle-walled carbon nanotubes (SWCNTs)en_US
dc.titleBioengineering a glucose oxidase nanosensor for near-infrared continuous glucose monitoring†en_US
dc.type.csemresearchareasTools for Life Sciencesen_US

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CC0 1.0 Universal
Except where otherwise noted, this item's license is described as CC0 1.0 Universal