Point-of-Care Fluorescence Biosensing System for Rapid Multi-Allergen Screening

dc.contributor.authorDemuru, Silvia
dc.contributor.authorChai-Gao, Hui
dc.contributor.authorShynkarenko, Yevhen
dc.contributor.authorHermann, Nicola
dc.contributor.authorBoia, Patricia
dc.contributor.authorCristofolini, Peter
dc.contributor.authorPetkus, Bradley
dc.contributor.authorGenerelli, Silvia
dc.contributor.authorPaoletti, Samantha
dc.contributor.authorCattaneo, Stefano
dc.contributor.authorBurr, Loic
dc.date.accessioned2024-05-22T07:56:33Z
dc.date.available2024-05-22T07:56:33Z
dc.date.issued2024-05-21
dc.description.abstractWith the steady increase in allergy prevalence worldwide, there is a strong need for novel diagnostic tools for precise, fast, and less invasive testing methods. Herein, a miniatured fluorescence-based biosensing system is developed for the rapid and quantitative detection of allergenspecific immunoglobulin-E. An antibody-based fluorescence assay in a microfluidic-patterned slide, combined with a custom-made portable fluorescence reader for image acquisition and user-friendly software for the data analysis, enables obtaining results for multiple allergens in just ~1 h with only 80 µL of blood serum. The multiplexed detection of common birch, timothy grass, cat epithelia, house dust mite, and dog epithelia shows quantitative IgE-mediated allergic responses to specific allergens in control serum samples with known total IgE concentration. The responses are verified with different control tests and measurements with a commercial fluorescence reader. These results open the door to point-of-care allergy screening for early diagnosis and broader access and for largescale research in allergies.
dc.description.sponsorshipThis project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No 874864 HEDIMED.
dc.identifier.citationhttps://doi.org/10.3390/s24113280
dc.identifier.urihttps://hdl.handle.net/20.500.12839/1395
dc.language.isoen
dc.titlePoint-of-Care Fluorescence Biosensing System for Rapid Multi-Allergen Screening
dc.typeArticle
dc.type.csemdivisionsBU-R
dc.type.csemresearchareasTools for Life Sciences
dc.type.csemresearchareasPhotonics
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