Challenges and perspectives of CRISPR-based technology for diagnostic applications
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Author
Del Giovane, Ste
Neda, Bagheri
Di Pede, A. C.
Chamorro, A.
Ranallo, S.
Migliorelli, D.
DOI
https://doi.org/10.1016/j.trac.2024.117594
Abstract
The precision and versatility of CRISPR-based techniques, combined with the advantages of nucleic acid-based
nanotechnology, hold great promise in transforming the landscape of molecular diagnostics. While significant
progress has been made, current CRISPR-based platforms primarly focus on nucleic acid detection. To expand the
applicability and fully leverage the advantages offered by CRISPR-based diagnostics, ongoing efforts explore
molecular strategies to develop CRISPR sensors capable of detecting a diverse range of analytes beyond nucleic
acids. In addition, challenges still persist in the adaptation of CRISPR platforms for point-of-care (POC) applications,
involving concerns such as portability and automation, as well as the complexities associated with
multiplexing. Here, we provide a detailed classification and comprehensive discussion of molecular strategies
facilitating the conversion of non-nucleic acid target binding into CRISPR-powered outputs with an emphasis on
their corresponding design principles. Furthermore, the second part of the review outlines current challenges and
potential solutions for seamlessly integrating these strategies into user-friendly platforms and rapid tests specifically
tailored for point-of-care (POC).
Publication Reference
TrAC Trends in Analytical Chemistry, Volume 172 (2024), 117594
Year
2024-03