Challenges and perspectives of CRISPR-based technology for diagnostic applications

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Del Giovane, Ste
Neda, Bagheri
The precision and versatility of CRISPR-based techniques, combined with the advantages of nucleic acid-based nanotechnology, hold great promise in transforming the landscape of molecular diagnostics. While significant progress has been made, current CRISPR-based platforms primarly focus on nucleic acid detection. To expand the applicability and fully leverage the advantages offered by CRISPR-based diagnostics, ongoing efforts explore molecular strategies to develop CRISPR sensors capable of detecting a diverse range of analytes beyond nucleic acids. In addition, challenges still persist in the adaptation of CRISPR platforms for point-of-care (POC) applications, involving concerns such as portability and automation, as well as the complexities associated with multiplexing. Here, we provide a detailed classification and comprehensive discussion of molecular strategies facilitating the conversion of non-nucleic acid target binding into CRISPR-powered outputs with an emphasis on their corresponding design principles. Furthermore, the second part of the review outlines current challenges and potential solutions for seamlessly integrating these strategies into user-friendly platforms and rapid tests specifically tailored for point-of-care (POC).
Publication Reference
Del Giovane, S., Bagheri, N., Di Pede, A. C., Chamorro, A., Ranallo, S., Migliorelli, D., ... & Porchetta, A. (2024). Challenges and perspectives of CRISPR-based technology for diagnostic applications. TrAC Trends in Analytical Chemistry, 117594.