PHOTOCHEMICAL LINKAGE OF ANTIBODIES TO SILICON CHIPS
Antibodies and antigen binding fragments thereof were photochemically immobilized on surface-modified silicon chips of 5 × 5 mm size. Silicon surface-grafted diazirines and benzophenones formed covalent bonds with the immunoreagents on light activation. Photolithographic immobilization of monoclonal antibodies in aqueous media was achieved on silicon chips by activating surface-grafted benzophenones. The presence of bovine serum albumin during irradiation reduced nonspecific adsorption of the immunoreagents and retained the immunoactivity of the photoimmobilized molecules.
J. Photochem. Photobiol. (1995) 6, 540-544