A Three-Dimensional Engineered Cardiac In Vitro Model: Controlled Alignment of Cardiomyocytes in 3D Microphysiological Systems

Simple item page
dc.contributor.authorNavaee, Fatemeh
dc.contributor.authorKhornian, Niloofar
dc.contributor.authorLonget, David
dc.contributor.authorHeub, Sarah
dc.contributor.authorBoder-Pasche, Stéphanie
dc.contributor.authorWeder, Gilles
dc.contributor.authorKleger, Alexander
dc.contributor.authorRenaud, Philippe
dc.contributor.authorBraschler, Thomas
dc.date.accessioned2023-02-17T12:00:59Z
dc.date.available2023-02-17T12:00:59Z
dc.date.issued2023-02-10
dc.description.abstractCardiomyocyte alignment in myocardium tissue plays a significant role in the physiological, electrical, and mechanical functions of the myocardium. It remains, however, difficult to align cardiac cells in a 3D in vitro heart model. This paper proposes a simple method to align cells using microfabricated Polydimethylsiloxane (PDMS) grooves with large dimensions (of up to 350 m in width), similar to the dimensions of trabeculae carneae, the smallest functional unit of the myocardium. Two cell groups were used in this work; first, H9c2 cells in combination with Nor10 cells for proof of concept, and second, neonatal cardiac cells to investigate the functionality of the 3D model. This model compared the patterned and nonpatterned 3D constructs, as well as the 2D cell cultures, with and without patterns. In addition to alignment, we assessed the functionality of our proposed 3D model by comparing beating rates between aligned and non-aligned structures. In order to assess the practicality of the model, the 3D aligned structures should be demonstrated to be detachable and alignable. This evaluation is crucial to the use of this 3D functional model in future studies related to drug screening, building blocks for tissue engineering, and as a heart-on-chip by integrating microfluidics.en_US
dc.identifier.citationCells 2023, 12, 576en_US
dc.identifier.doihttps://doi.org/10.3390/cells12040576
dc.identifier.urihttps://hdl.handle.net/20.500.12839/1158
dc.language.isoenen_US
dc.rightsCC0 1.0 Universal*
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.subject3D cell cultureen_US
dc.subject3D hydrogelen_US
dc.subjectin vitro cardiac modelen_US
dc.subjectcardiac cell alignmenten_US
dc.subjectdecellularized extracellular matrix (ECM)en_US
dc.subjectmicrofabricated groovesen_US
dc.titleA Three-Dimensional Engineered Cardiac In Vitro Model: Controlled Alignment of Cardiomyocytes in 3D Microphysiological Systemsen_US
dc.typeJournal Articleen_US
dc.type.csemdivisionsBU-Ten_US
dc.type.csemresearchareasTools for Life Sciencesen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Cells_A Three-Dimensional Engineered Cardiac In Vitro Model.pdf
Size:
2.39 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.82 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Research Publications