Continuous Flow Immunomagnetic Isolation of Circulating Rare Cells from Undiluted Whole Blood for Single-Cell Analysis
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Author
Blache, Manon
Coulon, Charlotte
Kurth, Felix
Eyer, Klaus
DOI
Abstract
Blood offers minimally invasive access to biomarkers, including rare circulating cells like Hematopoietic Stem Cells or Circulating Tumor Cells. However, their very low abundance (<0.01% of total blood cells) makes them difficult to isolate. The system presented here uses continuous positive immunomagnetic enrichment to process undiluted whole blood without clogging, achieving 80% sensitivity and 95% purity at 100 µL/min. Isolated cells can then be compartmentalized using droplet microfluidics for high-resolution single-cell analysis. The workflow is highly specific, adaptable to various targets, and applicable to liquid biopsies, prenatal testing, and endometriosis detection. It supports efficient rare cell isolation and multiomics analysis, advancing precision diagnostics and therapeutic development.
Publication Reference
Physics and Chemistry of Microfluidics GRC, Lucca, Italy
Year
2025-06-01
Sponsors
Biosystems Engineering Group, CSEM SA Centre Suisse d’Electronique et de Microtechnique, Neuchâtel, Switzerland
Department of Chemistry and Applied Biosciences, ETH Zürich, Zürich, Switzerland
EPFL School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
Department of Biomedicine, Aarhus University, Aarhus, Denmark